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. 2012 Nov 26;109(50):20614–20619. doi: 10.1073/pnas.1215765109

Fig. 3.

Fig. 3.

Wnt7a ameliorates the muscle phenotype in mdx mice. (A) Injection of a single dose of recombinant Wnt7a into TA muscles of mdx mice leads to a decrease in IgG-positive fibers at 3 wk after injection. n = 4. *P < 0.05. (B and C) Representative images of IgG staining (green) and laminin (red) of TA muscles from mdx mice injected with recombinant Wnt7a (B) or BSA as a control (C). Nuclei are counterstained with DAPI (blue). (Scale bar: 250 μm.) (D) Wnt7a reduces the number of fibers with centrally located nuclei. n = 4. **P < 0.01. (E–H) Representative images of mdx muscles electroporated with a Wnt7a expression plasmid (E and F) or a control plasmid (G and H). Pax7 staining is shown in green (E and G); laminin staining, in red (F and H). Nuclei are counterstained with DAPI (blue). (Scale bar: 100 μm.) (I) Wnt7a increases the number of satellite cells (marked by Pax7 expression) of TA muscles from mdx mice. n = 4. ***P < 0.001. (J) Wnt7a increases the number of satellite cells marked by the expression of Pax7 (MyoD-positive and -negative), but does not increase the numbers of myoblasts (Pax7-negative, MyoD-positive). n = 4.