Figure 3.

T-cells and Tregs in the peripheral blood of glioma patients and healthy donors. (A) Case control comparison of T-cells measured via CD3Z demethylation assay. (B) Case control comparison of Tregs measured via FOXP3 demethylation assay. (C) Case control comparison of Treg percent of T-cells determined by the ratio of FOXP3/CD3Z demethylation. In each panel, the displayed p value is from a Wilcoxon rank sum test between non-diseased control bloods and GBM patient bloods. Each data point represents the average of all replicate qMSP measurements for a single individual. Box plots superimposed on the data points cover the 2nd and 3rd quartile range with a line drawn at the median value, and whiskers that extend to the data point that is no more than 1.5 times the length of the box away from the box. The notches surrounding each median line extend to ± 1.58 IQR/sqrt(n), such that if the notches from two boxplots do not overlap there is strong evidence for a significant difference in the two medians.