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. 2012 Dec 21;7(12):e52701. doi: 10.1371/journal.pone.0052701

Figure 7. Expression of miR-133b, pitx3 and its target genes (th, dat, drd2a and drd2b) in zebrafish embryos microinjected with the Pitx3-3'UTR sequence at 24 hpf.

Figure 7

Expression of miR-133b (A), pitx3 (B), th (C), dat (D), drd2a (E) and drd2b (F) in zebrafish embryos microinjected with the Pitx3-3'UTR. Two hundred and fifty embryos were used for RNA extraction and cDNA synthesis in each group. In all cases, mRNA expression of pitx3, drd2a and drd2b was normalized to the expression of ef1α, which was taken as the reference gene. A minimal variation in ef1α (Fig. 7G) expression was found in the 3'UTR-Pitx3 microinjection group with respect to the control group. Each bar represents the number of cDNA copies of the genes studied ± SEM. For each group, three experiments were performed, each in triplicate. P values were calculated with the two-tail unpaired Student's t test. *P<0.05, **P<0.01 and ***P<0.001.