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. 2012 Dec 21;7(12):e52811. doi: 10.1371/journal.pone.0052811

Table 1. Primers used to amplify and sequence P53, Card 11, CD79B, MYD88 AND RPMS1 genes.

Primers Sequence Ta° Amplicon lenght
P53-1-F AAGTCTAGAGCCACCGTCCA 55°C 771 bp
P53-1-R AAGTGTTTCTGTCATCCAAATACTC
P53-2-F AGCCAAGTCTGTGACTTGCA 55°C 851 bp
P53-2-R GGGGAACAAGAAGTGGAGAA
Card11-1-F AGATGCAACGGGAGCCTGGC 55°C 631 pb
Card11-1-R AGGTTAGCAGCTCCACGCGC
Card11-2-F GGCCAAGGACCTGCAACGCT 55°C 703 pb
Card11-2-R CCGCTCCACCTCCTCCAGCT
Card11-3-F GAGGCCCTGGAGGACAGGCA 55°C 690 pb
Card11-3-R TCCGCAGGAGCTAGGGCTGG
Card11-4-F TCCTGCCCTACCATCCGCCC 55°C 671 pb
Card11-4-R CAGCAGCTGGTGGCCCTCAC
Card11-5-F TCCCAGCTCACCCTGCTGGG 55°C 739 pb
Card11-5-R CCGAGATGATGCGGACCCGC
Card11-6-F CCCGTCTCTCGCGAGCAAGC 55°C 928 pb
Card11-6-R CGTCTGCTGGGGCAGCTCTG
CD79B-1-F GCCTCGGACGTTGTCACGGG 55°C 858 pb*
CD79B-1-R TGGGCCAGCTTCAGAGGCCA
MYD88-EX1F CGCAGGAGAAAGAGGAAGC 60°C 491 bp
MYD88-EX1R ATGGGAGACAGGATGCTGAG
MYD88-EX3F TGGGTAAAGAGGTAGGCACTCCCAG 60°C 275 bp
MYD88-EX2R GCCCATCTGCTTCAAACACCCATGC
MYD88-EX3F AAGCCTTCCCATGGAGCTCTGACCAC 60°C 311 bp
MYD88-EX3R GCTAGGAGGAGATGCCCAGTATCTG
MYD88-EX3F ACTAAGTTGCCACAGGACCTGCAGC 60°C 194 bp
MYD88-EX3R ATCCAGAGGCCCCACCTACACATTC
MYD88-EX3F GTTGTTAACCCTGGGGTTGAAG 60°C 297 bp
MYD88-EX3R GCAGAAGTACATGGACAGGCAGACAGATAC
RPMS1F GGATGGGAGAGGGTGATCTT 60°C 151 bp
RPMS1R ACGTGGAGTTTGCAGTCCTC

Isoform 1: 858 pb; Isoform 2: 546 pb; Isoform 3: 861 pb.