Table 1. Primers used to amplify and sequence P53, Card 11, CD79B, MYD88 AND RPMS1 genes.
Primers | Sequence | Ta° | Amplicon lenght |
P53-1-F | AAGTCTAGAGCCACCGTCCA | 55°C | 771 bp |
P53-1-R | AAGTGTTTCTGTCATCCAAATACTC | ||
P53-2-F | AGCCAAGTCTGTGACTTGCA | 55°C | 851 bp |
P53-2-R | GGGGAACAAGAAGTGGAGAA | ||
Card11-1-F | AGATGCAACGGGAGCCTGGC | 55°C | 631 pb |
Card11-1-R | AGGTTAGCAGCTCCACGCGC | ||
Card11-2-F | GGCCAAGGACCTGCAACGCT | 55°C | 703 pb |
Card11-2-R | CCGCTCCACCTCCTCCAGCT | ||
Card11-3-F | GAGGCCCTGGAGGACAGGCA | 55°C | 690 pb |
Card11-3-R | TCCGCAGGAGCTAGGGCTGG | ||
Card11-4-F | TCCTGCCCTACCATCCGCCC | 55°C | 671 pb |
Card11-4-R | CAGCAGCTGGTGGCCCTCAC | ||
Card11-5-F | TCCCAGCTCACCCTGCTGGG | 55°C | 739 pb |
Card11-5-R | CCGAGATGATGCGGACCCGC | ||
Card11-6-F | CCCGTCTCTCGCGAGCAAGC | 55°C | 928 pb |
Card11-6-R | CGTCTGCTGGGGCAGCTCTG | ||
CD79B-1-F | GCCTCGGACGTTGTCACGGG | 55°C | 858 pb* |
CD79B-1-R | TGGGCCAGCTTCAGAGGCCA | ||
MYD88-EX1F | CGCAGGAGAAAGAGGAAGC | 60°C | 491 bp |
MYD88-EX1R | ATGGGAGACAGGATGCTGAG | ||
MYD88-EX3F | TGGGTAAAGAGGTAGGCACTCCCAG | 60°C | 275 bp |
MYD88-EX2R | GCCCATCTGCTTCAAACACCCATGC | ||
MYD88-EX3F | AAGCCTTCCCATGGAGCTCTGACCAC | 60°C | 311 bp |
MYD88-EX3R | GCTAGGAGGAGATGCCCAGTATCTG | ||
MYD88-EX3F | ACTAAGTTGCCACAGGACCTGCAGC | 60°C | 194 bp |
MYD88-EX3R | ATCCAGAGGCCCCACCTACACATTC | ||
MYD88-EX3F | GTTGTTAACCCTGGGGTTGAAG | 60°C | 297 bp |
MYD88-EX3R | GCAGAAGTACATGGACAGGCAGACAGATAC | ||
RPMS1F | GGATGGGAGAGGGTGATCTT | 60°C | 151 bp |
RPMS1R | ACGTGGAGTTTGCAGTCCTC |
Isoform 1: 858 pb; Isoform 2: 546 pb; Isoform 3: 861 pb.