Table 2. Marker expression by VR09 cell line and tumor masses, as assessed by immunohistochemistry performed on FFPE cell block.
Antibody | Species | Clone | Source | Diluition | VR09 cell line | Tumor masses |
Annexin a1 | mouse | B01P | Abnova | 100 | neg | neg |
Bcl-2 | mouse | 124 | Dako | 40 | pos | pos |
Bcl-6 | mouse | LN22 | Novocastra | 20 | neg | neg |
Cyclin D1 | rabbit | SP4 | Neomarkers | 10 | neg | neg |
CD03 | rabbit | SP7 | Labvision | 150 | neg | neg |
CD05 | mouse | 4C7 | Novocastra | 200 | neg | neg |
CD10 | mouse | 56C6 | Novocastra | 50 | neg | neg |
CD19 | mouse | LE-CD19 | Serotec | 200 | pos | pos |
CD20 | mouse | L26 | Novocastra | 100 | pos | pos |
CD22 | mouse | FPC1 | Novocastra | 20 | pos | pos |
CD23 | mouse | 1B12 | Novocastra | 100 | pos | pos |
CD25 IL2R | mouse | 4C9 | Novocastra | 100 | pos/neg | pos/neg |
CD38 | mouse | 38C03 | Neomarkers | 50 | neg | pos/neg |
CD43 (T cell) | mouse | MT1 | Novocastra | 30 | pos | pos |
CD79alfa | mouse | JCB117 | Dako | 100 | pos | pos |
CD79beta | mouse | JS01 | Novocastra | 50 | pos/neg | pos/neg |
CD123 | mouse | 7G3 | Bd | 100 | neg | pos/neg |
CD138 (syndecan-1) | mouse | BB4 | Serotec | 50 | pos/neg | pos/neg |
DBA44 hairy cell | mouse | DBA.44 | Dako | 20 | neg | neg |
FOXP1 | mouse | JC12 | Abcam | 500 | neg | pos/neg |
EBV (EBER) | mRNA probe* | Vision Byosistems | pos | pos | ||
GCET1 | mouse | RAM341 | Abcam | 500 | neg | neg |
HHV-8 | mouse | 13B10 | Novocastra | 50 | neg | neg |
IgD | rabbit | polyclonal | Dako | 20 | pos | pos/neg |
IgG | rabbit | polyclonal | Dako | 10000 | neg | neg |
IgM | rabbit | polyclonal | Dako | 5000 | pos/neg | pos/neg |
Ki-67 | mouse | MM1 | Novocastra | 20000(15′ incubation) | pos(40%) | pos(80%) |
ZAP-70 | mouse | 2F3.2 | Upstate | 200 | neg | neg |
κ chains | rabbit | polyclonal | Dako | 50 | pos | pos |
λ chains | rabbit | Dako | 30000 | neg | neg | |
MNDA | mouse | 235 | provided by CNIO** | 2 | pos | pos |
MUM1 Protein | mouse | MUM1p | Dako | 50 | pos | pos |
PAX 5 | mouse | 1EW | Novocastra | 50 | pos/neg | pos/neg |
P53 Protein | mouse | DO-7 | Novocastra | 20 | pos/neg | pos/neg |
Sox11 | rabbit | polyclonal | Atlas Sigma | 50 | pos/neg | pos/neg |
TCL1A | mouse | 1–21 | Santa Cruz | 100 | pos/neg | pos/neg |
TdT | rabbit | polyclonal | Dako | 30 | neg | neg |
TRAcP | mouse | 26E5 | Neomarkers | 30 | neg | neg |