Figure 5. Virus replication in various tissues of EV71-infected gerbils.

Twelve 21-day-old gerbils were inoculated EV71 IP with a dose of 1×10^5.0TCID₅₀ and tissue samples were collected at the time points indicated (3 gerbils/each). Virus titer in culture (solid bars) was expressed as TCID₅₀ per milliliter (blood) or per gram (tissue). Calculated virus titer (hatched bars) was based on standard curve of real-time RT-PCR obtained with 10-fold serial dilutions of virus strain 58301. The data shown here are the mean virus titers ±standard errors (n = 3 each). Results are representatives of two independent experiments. The data between the virus titer in culture and calculated virus titer were no statistically significant by student’s t-test.