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. 2012 Dec 21;7(12):e52010. doi: 10.1371/journal.pone.0052010

Figure 4. Zymosan induces the nuclear translocation of the NF-κB subunits cRel, RelA and p50 in RAW 264.7 macrophages.

Figure 4

Super-shift assays with anti-cRel, anti-RelA and anti-p50 antibodies using nuclear extract protein (8 µg) from RAW 264.7 macrophages in the presence (10 µg/mL, 15 min) or absence of zymosan. C1-3 represents nuclear protein-NF-κB double-stranded oligonucleotide complexes.