Figure 8. Cycloheximide enhances the rate of disappearance of CNBP-positive foci.

(A and B) To induce P-bodies and stress granules, HeLa cells expressing RFP-DCP1a and RFP-RCK and untransfected cells were incubated with 500 µM arsenite for 30 minutes. Following arsenite treatment, cells were allowed to recover in fresh medium for 45 minutes in the (A) absence or (B) presence of cycloheximide. After fixation, transfected cells were subjected to immunofluorescence to detect CNBP (top and middle panels) or CNBP and TIAR (bottom panels) and examined using confocal microscopy.