Figure 9. Depletion of CNBP does not affect stress granule formation.

(A) siRNAs against CNBP, HRI or nontarget siRNAs were transfected into HeLa cells. After 72 hours, cells were subjected to immunofluorescence to detect DCP1a and TIAR. (B) Histogram showing the percentage of cells with P-bodies and stress granules before and after arsenite induction. P-bodies and stress granules were visualized by performing immunofluorescence with anti-DCP1a and anti-TIAR antibodies, respectively. Data are from three independent experiments. (C) The efficiency of CNBP depletion was monitored by Western blotting. GAPDH was used as a loading control. (D) The efficiency of HRI mRNA depletion was assayed using quantitative RT-PCR.