Figure 1. Riboflavin and pyridoxal decrease EGFP photostability.

Graphs show bleaching of EGFP fluorescence in live HEK293T cells in DMEM^gfp (DMEM lacking vitamins) (black squares) or in DMEM^gfp supplemented with riboflavin (A, green circles) or pyridoxal (B, blue triangles). Fluorescence intensities in individual cells are background subtracted and normalized to maximum (initial) value in each cell. Standard deviation values (n = 15–20 cells in a representative experiment out of three independent experiments) are shown.