Figure 2. Fluorescence microscopy of live HEK293T cells transiently expressing EGFP.

Representative raw images of cells in green channel used to compose graphs in Figure 1A and B are shown. Cells were imaged in DMEM^gfp (left), DMEM^gfp with pyridoxal (middle), and DMEM^gfp with riboflavin (right) before (upper row) and after (bottom row) 75 s bleaching with intense blue light. Scale bar 20 µm.