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. 2012 Dec 21;7(12):e52091. doi: 10.1371/journal.pone.0052091

Figure 5. Anti-flagella antibodies block the secretion of pro-inflammatory cytokines.

Figure 5

Purified flagella (100 pg/ml) and flagellin (100 ng/ml) were pre-incubated with anti-flagella antibodies in LPS-free water before the addition to macrophage cells. The antibodies were tested undiluted and using different dilutions (1∶10, 1∶100, and 1∶200). (A) IL-8 release was inhibited when flagella (94, 88, 48 and 48%) and flagellin (96, 94, 52 and 51%) were incubated with different dilutions of anti-flagella. (B and C) TNF-α and IL-10 releases were blocked completely with the three dilutions and with the undiluted antibody. RPMI-1640 medium.