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. 2012 Dec 21;7(12):e52091. doi: 10.1371/journal.pone.0052091

Figure 7. Transfection assay in HEK293-hTLR5 cells enables IL-8 secretion in response to C. sakazakii flagella and flagellin.

Figure 7

293-hTLR5 cells (HEK293 cells transfected with TLR5) secreted 197 pg/ml and 133 pg/ml of IL-8 after treatment with C. sakazakii (ST1) flagella and flagellin, respectively; and 196 pg/ml of IL-8 were released with FliC-Salmonella enterica serovar Typhimurium. 293-hTLR5 cells pre-incubated with anti-hTLR5-IgA antibodies at a concentration of 10 µg/ml showed a reduction of 76% in IL-8 release.