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. 2012 Dec 21;7(12):e52164. doi: 10.1371/journal.pone.0052164

Figure 6. Effect of simultaneous resveratrol and L-NAME treatment and SNP on ammonia-induced S100B secretion in C6 astroglial cells.

Figure 6

Cells were pre-treated with 100 µM resveratrol (RSV) and 500 µM L-NAME simultaneously, in the presence of 5 mM ammonia for 24 h (A). Cells were pre-treated for 1 h with 100 µM resveratrol (RSV) or 500 µM L-NAME, followed by incubation with 40 µM SNP for 24 h (B). The basal secretion level, assumed to be 100%, is indicated by the line. Data represent means ± S.E.M of three experimental determinations performed in triplicate, analyzed statistically by two-way ANOVA followed by the Tukey’s test. (a) indicates significant differences from the control (P<0.05). (b) indicates significant differences from ammonia (P<0.05). (c) indicates significant differences from SNP (P<0.05).