Figure 4. APO-IL-15 and IL-15Rα sushi domain gene co-transfer induces proliferation of adoptively transferred CD8 and NK lymphocytes.

Mice were gene-transferred by hydrodynamic injections of the indicated plasmids which were 24 h later intravenously injected with CD8⁺ T cells or NK1.1⁺ NK cells preloaded with CFSE. Proliferation was monitored by CFSE dilution of the indicated electronically gated lymphocyte subsets (dot plots A and C). Results pooled from two independent experiments included two mice per experimental group (B and D). Figure S7 shows proliferative effects on endogenous lymphocytes studied by BrdU intake.