Figure 3. Atg9 is inhibitory for CPP-induced autophagy.

Atg9 wild type and Atg9-deficient MEFs stably expressing GFP-LC3 were treated with EBSS or CPP followed by Western blotting analysis (A) and quantification of GFP-LC3 puncta (B–D). Scale bars: 10 µm in B. Western blots shown are from one representative experiment of two to three performed for each panel. Quantitative data (mean±SEM) are from 3–5 experiments. *: P<0.05, **: P<0.01.