. Author manuscript; available in PMC: 2013 Jun 1.

Published in final edited form as: J Antibiot (Tokyo). 2012 Oct 10;65(12):615–622. doi: 10.1038/ja.2012.81

Table 2.

Physico-chemical properties of Pyramidamycin D (5) and 3-Hydroxyquinoline-2-carboxamide (6).^a)

	Pyramidamycin D (5)	3-Hydroxyquinoline-2-carboxamide (6)
Appearance	White powder, UV absorbing	Pale yellow solid, UV absorbing, green fluorescence under long UV (365 nm)
Anisaldehyde/Sulfuric acid ^b)	Pale yellow	-
R_f	0.31 (CH₂Cl₂/7%MeOH)	0.30 (CH₂Cl₂)
Molecular formula	C₁₀H₁₂N₂O₄	C₁₀H₈N₂O₂
(−)-ESI MS: m/z	223 [M−H]⁻	187 [M−H]⁻
(+)-ESI MS: m/z	225 [M+H]⁺	189 [M+H]⁺
(+)-HRESI MS (m/z)
Found	225.0875 [M+H]⁺, 247.0704 [M+Na]⁺ and 263.0438 [M+K]⁺	189.0653 [M+H]⁺ and 211.0473 [M+Na]⁺
Calcd.	225.0870 for C₁₀H₁₃N₂O₄, 247.0689 for C₁₀H₁₂N₂O₄Na and 263.0429 for C₁₀H₁₂N₂O₄K	189.0658 for C₁₀H₉N₂O₂ and 211.0478 for C₁₀H₈N₂O₂Na
UV/VIS (MeOH): λ_max (log ε)	235 (4.13), 271 (3.84), 297 (3.63) nm.	218 (4.18), 231 (4.24), 296 (4.59), 358 (3.58) nm.

^a)

^b)

Colouration with anisaldehyde/sulfuric acid spraying reagent and heating.