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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Cancer. 2012 Sep 18;119(4):774–781. doi: 10.1002/cncr.27742

Figure 2.

Figure 2

Chrysin activated Notch1 signaling in both HTh7 and KAT18 cell lines. To determine whether chrysin activated the Notch pathway, ATC cells were first transiently transfected with CBF1-luciferase reporter constructs and then treated with various concentrations of chrysin for 48 hours. Luciferase activity was measured and normalized to β-galactosidase units and expressed as relative light unit (RLU). Chrysin treatment resulted in a significant increase of RLU in ATC cells (a). Total cell lysates were isolated from both HTh7 and KAT18 48 hours after chrysin treatment. Protein expression of Notch1 intracellular domain (NICD) and Hes1, a Notch1 down-stream target, were both increased with the treatment as demonstrated by Western blot (b). Additionally, relative expression levels of Notch1 mRNA were induced with chrysin treatment (c), as well as the levels of its target gene, Hes1 (d). All values were expressed as mean ± SEM (n=3, *p<0.05 and **p<0.01 compared with control).