. Author manuscript; available in PMC: 2013 Dec 21.

Published in final edited form as: ACS Chem Biol. 2012 Sep 26;7(12):1994–2003. doi: 10.1021/cb300385m

Table 1.

Substrate preferences of CurM ST and OLS ST

	Percent sulfonated^a

	CurM ST	OLS ST

(R)-3-hydroxydodecanoyl-ACP	73 ± 3	20.4 ± 0.3
(R)-3-hydroxytetradecanoyl-ACP	72 ± 2	33 ± 1
(3R)-3-hydroxy-5-methoxytetradecanoyl-ACP	74 ± 5	0
(3S)-3-hydroxy-5-methoxytetradecanoyl-ACP	28 ± 2	0

Raw HPLC chromatogram peak areas for the ACP-loaded substrate and sulfonated product were used to calculate the fraction of substrate sulfonated. CurM ST reactions were quenched and analyzed after 5 min and OLS ST after 3 h. Data are mean ± standard deviation from triplicate experiments.