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. Author manuscript; available in PMC: 2013 Jun 20.
Published in final edited form as: Nature. 2012 Nov 14;492(7429):443–447. doi: 10.1038/nature11709

Figure 3. Tet1 activates meiotic genes through DNA demethylation.

Figure 3

(a) Scatter plot comparing transcriptome of wild-type and Tet1Gt/Gt E13.5 female PGCs. There are 111 and 899 genes that are respectively up- or down-regulated (FDR<0.05). Examples of down-regulated meiotic genes include Mael, Sycp3, Stra8, Sycp1, and Prdm9 indicated.

(b) Gene ontology analysis of down-regulated genes in Tet1Gt/Gt PGCs with cutoff FDR<0.05. The most enriched biological processes based on their p-values are shown.

(c) ChIP-qPCR analysis of E13.5 wild-type female PGCs using anti-Tet1 antibody demonstrates binding of Tet1 to the promoters of Sycp1, Mael, and Sycp3. Upper panels, diagrams of Sycp1, Mael, and Sycp3 genes with the analyzed regions indicated by red lines. Bottom panels, relative enrichment of Tet1 over IgG control. n=3. Error bars indicate SEM. *P<0.05. **P<0.01.