Table 1.
Outcomes of oocyte quality and embryo development across the FF L:A ratio tertiles
| Embryological outcomes | Low FF L:A | Med FF L:A | High FF L:A | All | P |
|---|---|---|---|---|---|
| Age (years) | 37.6 ± 5.1 | 35.6 ± 4.0 | 36.4 ± 3.2 | 36.6 ± 4.2 | 0.555 |
| BMI (kg/m2) | 20.4 (4.4) | 23.4 (2.5) | 27.3 (13.3) | 23.4 (4.3) | 0.036 |
| Baseline serum AMH (ng/mL) | 2.4 (2.6) | 1.2 (1.2) | 2.3 (1.9) | 1.6 (2.0) | 0.530 |
| Peak serum estradiol (pg/mL) | 2384 ± 1033 | 1717 ± 792 | 2259 ± 1530 | 2114 ± 1113 | 0.339 |
| Oocytes studied N | 24 | 25 | 25 | 74 | |
| Mature (MII) oocytes N (% total) | 22 (91.7) | 23 (92.0) | 23 (92.0) | 68 | 0.796 |
| Normal fertilization (2PN) N (% MII) | 13 (59.1) | 16 (69.6) | 18 (78.3) | 47 | 0.223 |
| Successful cleavage N (% 2PN) | 6 (46.2) | 11 (68.8) | 15 (83.3) | 32 | 0.050 |
| Viable cleavage morphology N (% 2PN) | 8 (38.5) | 10 (62.5) | 15 (83.3) | 30 | 0.019 |
| Successful blastulation N (% 2PN) | 0 | 2 (33.3) | 11 (78.6) | 13a | 0.028a |
| Viable blastocyst morphology N (% 2PN) | 0 | 2 (33.3) | 2 (14.3) | 4a | 0.574a |
AMH = anti-mullerian hormone; FF = follicular fluid; the L:A ratio = leptin to adiponectin ratio; Med = medium; MII = meiosis II; 2PN = 2 pronuclei. Values are reported as mean ± standard deviation for age and peak serum estradiol, as median (interquartile range) for body mass index and baseline serum AMH, and as number and percent for all other rows. P values were calculated using the One-Way Anova for age and peak serum estradiol, the nonparametric median test for body mass index and baseline serum AMH, the extended Mantel-Haenszel Chi Square test for linear trend for oocyte and embryo outcomes. The FF L:A tertiles are separated by 3.71E−3 and 19.79E−3
aFrom 13 blastocyst-only cycles (37 total oocytes and 23 normally fertilized oocytes)