Fig. 4.

Immunolocalization of 5 methyl cytosine. Labeling index of 5 methyl cytosine (5-MEC) in testicular germ cells; diabetic (black square) and control group (white square) Spermatogonia (SP), preleptotene/zygotene (PL/Z), pachytene (PS), round spermatids (RS), and elongated spermatids (ES). a) 36 days interval and b) 72 days interval, ^ap < 0.05 compared to diabetic group. c) Photomicrographs showing the immunolocalization of 5 methyl cytosine (brown labeling) in testicular germ cells in diabetic-72 group (above), negative control (omission of the primary antibody) for the 5-MEC staining (below)