Table 1.

Sequences and concentrations of primers and probes used in this study.

Viral target	Target region/amplicon size	Primer/probe name	Sequencesa	Final conc. (μM)	Reference
RoV	Non-structural protein 3 (NSP3)/87 bp	NVP3-FDeg	ACC ATC TWC ACR TRA CCC TC	1
		NVP3-R1	GGT CAC ATA ACG CCC CTA TA	1	Freeman et al. (2008)
		NVP3-Probe	FAM-ATG AGC ACA ATA GTT AAA AGC TAA CAC TGT CAA-BHQ1	0.125
NoVII	ORF1-ORF2 junction/98 bp	Cog 2F	CAR GAR BCN ATG TTY AGR TGG ATG AG	1
		Cog 2R	TCG ACG CCA TCT TCA TTC ACA	1	Trujillo et al. (2006)
		Ring 2	Cyan500–TGG GAG GGC GAT CGC AAT CT–BHQ1	0.125
NoVI	ORF1-ORF2 junction/84 bp	Cog 1F	CGY TGG ATG CGI TTY CAT GA	1
		Cog 1R	CTT AGA CGC CAT CAT CAT TYA C	1	Trujillo et al. (2006)
		Ring 1 C	FAM–AGA TYG CGI TCI CCT GTC CA–BHQ1	0.125
EAV		EAV-F	CAT CTC TTG CTT TGC TCC TTA G	0.2
		EAV-R	AGC CGC ACC TTC ACA TTG	0.2	Hue et al. (2011)
		EAV-probe	Cyan500–CGC GCT CGC TGT CAG AAC AAC ATT ATT GCC CAC AGC GCG–BHQ3	0.05

^aW = T, U, A; R = A, G; Y = C, T; B = C, G, T; N = any; I = inosine.