Fig. 6.

VEGF secretion and VEGFR-2 expression by HAEC. VEGF secretion and VEGFR-2 expression was determined after HAEC's stimulation with a 24 h rhCRP (25 μg/ml) treatment and a 1 h E2 pre-treatment (10⁻⁸ and 10⁻⁹ M) alone or in combination. VEGF was measured by ELISA in culture supernatants of HAEC (a). Cell surface VEGFR-2 expression was evaluated by flow cytometry and data are presented as MFI values (b). Data represent the mean ± SEM. N = 3 (a, b)