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. 2012 Sep 17;590(Pt 22):5749–5764. doi: 10.1113/jphysiol.2012.241299

Figure 6. The glial GlyT1 regulates extracellular glycine levels and glycine-dependent neurotransmitter release.

Figure 6

A, schematic of experimental design to test whether pre-incubation with GlyT antagonists or a gliotoxin blocks effects of glycine and strychnine. Antagonists/toxin were applied 60 min prior to the start of recordings. After a 10 min baseline period, glycine (gly) or strychnine (str) was applied for 10 min. B, example whole-cell recordings from L2/3 pyramidal cell bathed in sarcosine depicting miniature excitatory postsynaptic currents (mEPSCs) recorded at baseline and during bath application of 1 μm glycine (Ba) or 3 μm strychnine (Bb). C, averaged normalized mEPSC frequency demonstrates that pre-incubation with sarcosine, N-[3-(4′-fluorophenyl)-3-(4′-phenylphenoxy)propyl] sarcosine (NFPS) or d,l-α-aminoadipic acid (d,l-αAA), but not the GlyT2-specific antagonist Alx 1393, prevents the strychnine-induced reduction in mEPSC frequency. Pre-incubation with sarcosine, NFPS, Alx 1393 or d,l-αAA had no effect on glycine-dependent facilitation. D, averaged normalized mEPSC amplitude; no significant changes were observed with any of the pharmacological manipulations. The mEPSC frequencies and amplitudes in C and D were normalized to the averaged baseline levels before glycine or strychnine application.