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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Dev Growth Differ. 2012 Aug 23;54(7):673–685. doi: 10.1111/j.1440-169X.2012.01368.x

Fig. 2.

Fig. 2

Axolotl genomic DNA quantitative polymerase chain reaction (qPCR) analysis of the change of integrated LINE-1 gene copy number in limb regenerates from consecutive limb amputations and regenerations. Primers used for genomic DNA Real Time qPCR: D08, the set of primers for LINE-1 ORF-1. Unamputated: the unamputated limb. 1.regen: the limb regenerate from the first round of limb amputation performed on the unamputated limb; 2.regen: the limb regenerate regrown from the second round of limb amputation conducted in the 1.regen. Each of the four groups of bars represents the percentage change of genomic LINE-1 content in the unamputated limb, the 1.regen and the 2.regen in four individual animals, respectively.