Fig. 3.
EM images of negatively stained dynein constructs. Overview of samples (large images) and galleries of single dynein particles (small images): motor domain Dyn380kD (A); artificially dimerized motor domains cc-Dyn380kD (B); dynein complex (C); and CDHC (D). Scale is the same for all single particles images in A–D. (E) Histogram of the distribution of the Dyn380kD motor domain (MD) diameter [measured as indicated by red arrowheads in the EM image (30 × 30 nm)]. (F) Histograms of the distribution of the dynein complex MD diameter (Left) and the tail domain length (Right) [measured as indicated on the EM image (60 × 80 nm) by red and yellow arrowheads, respectively]. Indicated are mean values ± SD. d, diameter; l, length.