Table 2.

Summary of EC₅₀ and relative activity of parent and variant molecules on four CD22⁺ cell lines

Protein	CA46 (n = 5)		Raji (n = 5)		Daudi (n = 4)		HAL-01 (n = 4)
	EC50 ± SD (ng/mL)	Relative activity (%)	EC50 ± SD (ng/mL)	Relative activity	EC50 ± SD (ng/mL)	Relative activity (%)	EC50 ± SD (ng/mL)	Relative activity (%)
HA22	0.18 ± 0.09	100	0.15 ± 0.09	100	0.1 ± 0.05	100	1.14 ± 0.43	100
HA22-LR	0.41 ± 0.27	45	0.45 ± 0.18	33	0.11 ± 0.05	85	1.5 ± 0.45	76
HA22-L297A	0.32 ± 0.15	58	0.27 ± 0.13	54	0.12 ± 0.05	83	2.25 ± 0.61	51
HA22-Y298A	0.79 ± 0.52	23	0.47 ± 0.34	32	0.34 ± 0.15	28	6.7 ± 1.16	17