Table 2.
Numbers and types of gene conversions in 31 F2 plants
Sample | Direction of the gene conversion |
Total | ||
Col (%)→Het | Het→C or L | Ler (%)→Het | ||
5 | 106 (15.1) | 150 | 54 (14.0) | 310 |
6 | 27 (15.3) | 60 | 149 (22.9) | 236 |
7 | 62 (22.6) | 115 | 76 (18.7) | 253 |
14 | 61 (17.1) | 248 | 94 (18.5) | 403 |
c42 | 47 (39.8) | 27 | 46 (7.6) | 120 |
c45 | 77 (24.9) | 49 | 63 (11.5) | 189 |
c48 | 69 (23.7) | 40 | 92 (15.4) | 201 |
c51 | 70 (18.7) | 56 | 173 (23.6) | 299 |
c52 | 91 (24.4) | 55 | 82 (14.2) | 228 |
c57 | 6 (7.3) | 53 | 81 (12.0) | 140 |
c61 | 80 (56.3) | 50 | 16 (3.8) | 146 |
c62 | 134 (39.7) | 36 | 259 (13.7) | 429 |
c63 | 80 (21.7) | 54 | 405 (44.1) | 539 |
c64 | 61 (44.1) | 40 | 38 (7.9) | 139 |
c65 | 58 (19.2) | 67 | 82 (19.5) | 207 |
c66 | 45 (12.1) | 134 | 34 (5.3) | 213 |
c73 | 75 (21.8) | 154 | 15 (4.0) | 244 |
c81 | 31 (19.8) | 30 | 175 (42.3) | 236 |
c82 | 106 (22.5) | 45 | 236 (45.2) | 387 |
c83 | 81 (17.1) | 69 | 49 (12.7) | 199 |
c84 | 9 (7.4) | 59 | 159 (27.0) | 227 |
c85 | 141 (68.8) | 22 | 44 (5.8) | 207 |
c87 | 93 (27.6) | 44 | 222 (29.9) | 359 |
c88 | 59 (14.4) | 33 | 86 (20.8) | 178 |
c89 | 76 (19.6) | 122 | 146 (24.7) | 344 |
c90 | 82 (31.1) | 47 | 133 (16.8) | 262 |
c91 | 70 (21.1) | 29 | 470 (49.9) | 569 |
c92 | 55 (15.4) | 51 | 170 (30.6) | 276 |
c93 | 157 (47.1) | 39 | 42 (9.5) | 238 |
c94 | 34 (15.2) | 20 | 185 (54.2) | 239 |
c95 | 97 (30.2) | 35 | 75 (24.7) | 207 |
Average | 72.3 | 65.6 | 127.5 | 265.3 |
ABP | 0.252 | 0.540 | 0.208 | 1 |
S/C | 63/63 | 42/20 | 21/20 | 126/104 |
Estimated | 286.9 | 146.3 | 583.8 | — |
The gene conversion track length, the farthest distance between two or more markers, is 20–2,000 bp. Het, Col (C), and Ler (L) represent heterogeneous, Col- and Ler-homologous backgrounds, and their average background proportions (ABPs) are 0.540, 0.252, and 0.208, respectively. The individual proportions of Col and Ler background are shown in parentheses. The first number in S/C means the numbers of sampled gene conversions for PCR amplifications, and the second denotes the true gene conversions verified by Sanger sequencing. The estimated gene conversions are equal to the following: Average gene conversions × Confirmed/Sampled gene conversions/Proportion of corresponding background in each column.