Fig. 4.
Sex-specific expression of ascarosides in P. redivivus. (A) HPLC-MS/MS screen for precursor ions of m/z = 73 selectively detects ascarosides. (B) SIR for molecular ions of ascarosides detected by MS/MS in P. redivivus samples reveals sex-specific expression of ascarosides. Shown chromatograms are derived from mixed populations (black), females (green), and males (red). (C) Proposed biosynthetic pathway for ascaroside biosynthesis in P. redivivus. (D) Distribution of major ascaroside in male and female WW samples.