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. 2012 Dec 5;109(51):21022–21027. doi: 10.1073/pnas.1218925110

Fig. 1.

Fig. 1.

BCR-dependent association of PKN1 with Akt and cleavage. (A) Ramos cells, a human Burkitt’s lymphoma cell line, were stimulated with an antihuman IgM F(ab)2 for the indicated time periods. Cell lysates were immunoprecipitated (IP) with an anti-Akt or control antibody (shown as C) and immunoblotted as indicated. Whole cell extracts (WCE) correspond to 10% of the total cell lysates. (B) Ramos cells were stimulated with immobilized antihuman IgM F(ab)2 for 12 h. The cell lysates were immunoblotted with either an anti-PKN1 or anti-PKCδ antibody. Open arrowheads indicate full-length proteins. Closed arrowheads show the processed form of the proteins.