Fig. 5.

Global regulatory network that orchestrates B-cell fate. Network is based on the transcription signatures derived from EBF1- and FOXO1-deficient CLPs and genome-wide EBF1 and FOXO1 occupancy in pro-B cells. Intergenic feedback circuitry is indicated. Genes were selected with a significant change in transcript levels in EBF1- or FOXO1-ablated LY6D⁺ CLPs (at least a twofold change) and with a TSS proximal and distal (<250 kb) EBF1/FOXO1 occupancy. Genes fulfilling these criteria were divided into six groups: I, negatively regulated by EBF1; II, negatively regulated by both EBF1 and FOXO1; III, negatively regulated by FOXO1; IV, positively regulated by EBF1; V, positively regulated by EBF1 and FOXO1; VI, positively regulated by FOXO1.