Fig. 5.

Expression of the Pyl operon in A. arabaticum and D. dehalogenans. Total RNAs were extracted from cells and reversely transcribed using random hexamer primers. PCR amplifications were then performed from cDNA with primer pairs targeting individual genes as indicated. A positive control of each primer pair was performed on genomic DNA (gDNA). rpoB gene, encoding the beta subunit of the RNA polymerase, was used as internal control for amplification. (A) Gene expression was studied in A. arabaticum cells cultivated in the presence of Pyr or TMA. (B) Gene expression was studied in D. dehalogenans cells cultivated in the presence of Pyr.