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. 2012 Dec 3;109(51):21052–21057. doi: 10.1073/pnas.1216195109

Fig. 1.

Fig. 1.

Human hematopoietic cells are required to induce sepsis in NOD/SCID/IL2Rγ−/− mice. Mice underwent sham or CLP surgery and 24 h post-CLP, sera were tested for HMGB1 by ELISA (A), murine proinflammatory cytokines were tested by cytometric bead array (B), and human proinflammatory cytokines were tested by cytometric bead array (C). Mean ± SD from seven mice per group is shown. (D) Kaplan–Meier survival curves for mice following CLP are shown (n = 14 per group). (E) Representative hematoxylin and eosin (H&E)-stained sections from the liver taken 24 h after CLP from the indicated group of mice are shown. (F) Representative TUNEL-stained liver section (Left) and cumulative percentage of TUNEL+ cells determined by examining 20 high-powered fields from three mice in each group (Right) are shown. (G) Bacterial counts in the blood of indicated groups of mice on indicated days after CLP is shown (n = 5–7 per group). BALB/c, BLT, and neutrophil-depleted NOD/SCID/IL2Rγ−/− mice could not be serially followed because all mice died by day 2–3. (H) Neutrophil depletion was tested in blood 3 d after administration of Ly6G-specific mAb by staining for Ly6G and GR-1. (I) Kaplan–Meier survival curves for NOD/SCID/IL2Rγ−/− mice with intact or depleted neutrophils is shown (n = 5 per group). *P < 0.05.