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. 2012 Nov 28;109(51):20800–20807. doi: 10.1073/pnas.1218674109

Fig. 1.

Fig. 1.

Ats-1 binds Beclin 1 and triggers vesicle formation. (A–C) Co-IP assay for protein interaction between Ats-1 (without a tag) and HA-Beclin 1 (A), Ats-1(90–250)-GFP and HA-Beclin 1(1–272) (B), and Ats-1(218–376)-GFP and HA-Beclin 1(1–272) (C). Cotransfected HEK293 cells were immunoprecipitated (IP) with preimmune IgG or anti (α)–Ats-1. Precipitates were immunoblotted (IB) with anti-HA and anti–Ats-1. IgG, rabbit IgG heavy chain. (D) Ats-1–GFP forms clusters of vesicles of ∼0.5–1 μm in diameter in transfected RF/6A cells. (E) Localization of HA-Beclin 1 to vesicles containing tag-less Ats-1 in cotransfected RF/6A cells. (F) Ats-1–GFP vesicles are distinct from mitochondria labeled with anticytochrome c. (Scale bars: D–F, 10 μm.) Insets show magnified boxed areas.