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Cellular fractionation of Ap-infected cells. Immunoblotting of fractions (1–12) of Ap-infected HL-60 cells (I) and uninfected HL-60 cells (U) collected after OptiPrep density gradient centrifugation using anti–Ats-1 and various organelle markers: anti-LC3 (autophagosome), anticalnexin (ER), anti–LAMP-2 (lysosomes and late endosomes), anti-EEA1 (early endosomes), and anti-P44 (Ap inclusions).
