Table 1.

Translocation kinetics for wild-type and mutant γ-subunits

γ-Subunit	Half-time for translocation, s
	α2 adrenergic	CXCR4
γ9-E-KGGCLIS, wild type	9.0 ± 1.7 (22)	8.4 ± 1.2 (14)
γ9-EKKFFCLIS	16.5 ± 0.7 (31)	18.7 ± 1.4 (19)
γ3-EKKFFCALL, wild type	124 ± 17 (8)	129 ± 8 (23)
γ3-EKKAACALL	31.4 ± 1.7 (36)	34.2 ± 2.3 (23)
γ3-EAAFFCALL	37.8 ± 1.9 (29)	36.1 ± 1.6 (18)
γ3-EAAAACALL	20.6 ± 4.3 (22)	18.9 ± 4.4 (18)
γ3-EKKFFCLIS	15.7 ± 0.9 (26)	18.5 ± 0.6 (32)

Sequences correspond to the C-terminal regions of the subunits. Translocation half-times are given as the mean ± SEM (number of cells in parentheses) from at least three independent experiments. Residues of interest are in boldface: positively charged (red), hydrophobic (green), mutations (black, underlined).