Table 1.
γ-Subunit | Half-time for translocation, s |
|
α2 adrenergic | CXCR4 | |
γ9-E-KGGCLIS, wild type | 9.0 ± 1.7 (22) | 8.4 ± 1.2 (14) |
γ9-EKKFFCLIS | 16.5 ± 0.7 (31) | 18.7 ± 1.4 (19) |
γ3-EKKFFCALL, wild type | 124 ± 17 (8) | 129 ± 8 (23) |
γ3-EKKAACALL | 31.4 ± 1.7 (36) | 34.2 ± 2.3 (23) |
γ3-EAAFFCALL | 37.8 ± 1.9 (29) | 36.1 ± 1.6 (18) |
γ3-EAAAACALL | 20.6 ± 4.3 (22) | 18.9 ± 4.4 (18) |
γ3-EKKFFCLIS | 15.7 ± 0.9 (26) | 18.5 ± 0.6 (32) |
Sequences correspond to the C-terminal regions of the subunits. Translocation half-times are given as the mean ± SEM (number of cells in parentheses) from at least three independent experiments. Residues of interest are in boldface: positively charged (red), hydrophobic (green), mutations (black, underlined).