. 2012 Dec 3;109(51):E3568–E3577. doi: 10.1073/pnas.1205345109

Table 2.

In vitro membrane dissociation kinetics for peptides corresponding to the C-terminal domain of wild-type and mutant γ-subunits

Peptide sequence	Half-time for dissociation, s
Peptide sequence	PC/PE/PS, 20 °C	PC/PE/PS/Ch, 20 °C	PC/PE/PS/Ch, 37 °C
`EKGGC(farnesyl)-OMe`	0.20 ± 0.02	<0.14	<0.14
`EKFFC(farnesyl)-OMe`	0.74 ± 0.03	0.40 ± 0.05	0.30 ± 0.03
`KKFFC(farnesyl)-OMe`	7.0 ± 0.2	3.2 ± 0.2	2.4 ± 0.1
`EKGGC(geranylgeranyl)-OMe`	1.8 ± 0.2	1.1 ± 0.1	0.58 ± 0.04
`EKFFC(geranylgeranyl)-OMe`	59 ± 6	26 ± 1	12.5 ± 0.8
`KKFFC(geranylgeranyl)-OMe`	638 ± 24	368 ± 30	240 ± 21

Half-times are given as the mean ± SEM for three independent experiments. Membrane compositions: PC/PE/PS, 37.5/37.5/37.5; PC/PE/PS/cholesterol, 35/35/30/50.