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Left: pH titration curve for the 695 (heme-Met80, open circles) and 627 nm (heme-H2O, filled circles) absorption bands of hh cyt c. Right: Temperature profiles of the 695 nm absorbance at pH = 7.0 (open circles), 4.0 (filled squares), 3.5 (filled circles), and 3.0 (open squares). Inset show the baseline-corrected absorption spectra, as a function of pH and temperature (various colors).
