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pD titration curves for the 1408 cm−1 HisD2+ of hh cyt c at 20° C (circles), 40° C (squares) and 60° C (triangles). The insets show the aqueous histidine titration curve (upper) and the intensity-derived average protonation number as function of temperature(bottom). [The pH meter readings (pH*, in D2O) were converted to pD = 0.929×pH*+0.42 as described in ref. 61]
