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An example of hydrogen sulfide concentration determination in blood using C57BL6/J mice (n=5), determined using 96-well plates (DNS-Az 200 μM, excitation filter 360 nm, emission filter 528 nm). Zero point was obtained by trapping sulfide with ZnCl2 and calibration curve was obtained by using an internal standard method. (Hydrogen sulfide 0, x, x+10, x+50 and x+100 μM)
