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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: J Immunol. 2012 Nov 16;189(12):5682–5693. doi: 10.4049/jimmunol.1201966

Figure 3. Increased production of IL17 by Blimp-1-deficient CD4+ T cells in mixed-bone marrow chimeric mice.

Figure 3

Wild type CD45.1-irradiated mice received a total of ~4 × 106 HSC-enriched mixed BM cells from Ctrl (CD451.1) and Blimp-1CKO (CD451.2) mice. Eleven weeks after bone marrow cells transfer, mLN, SP and LI-LP cells were isolated from chimeric mice, stimulated as described in Fig. 1D and IL17 (A) production was analyzed in TCRβ+CD4+ cells in the CD45.2− (Ctrl) or CD45.2+ (Blimp1CKO) gates. B, Frequency of IFNγ-producing TCRβ+CD4+ cells from the MLN and SP from chimeric mice (stimulation and analysis done as in A). C) Frequency of Foxp3-expressing cells in the Ctrl and Blimp-1CKO compartments in the SP and mLN from chimeric mice (gated as shown in A). Plots (A–C) show compiled results from four to nine different chimeric mice.