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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: J Immunol. 2012 Nov 16;189(12):5682–5693. doi: 10.4049/jimmunol.1201966

Figure 6. Blimp-1 expression is induced by Th1 and Th2-inducing cytokines but repressed by TGFβ in Th17-developing cells.

Figure 6

A) Naïve CD4+ T cells from Blimp-1 reporter mice were stimulated with plate-bound anti-CD3 plus anti-CD28 alone or in the presence of the indicted cytokines and YFP (Blimp-1) expression was analyzed by FACS at d5. B) qRT-PCR analysis of Blimp-1 mRNA expression in the same cells from experiment shown in (A). C) Dose-dependent inhibition of Blimp-1 expression by TGFβ in Blimp-1 reporter naïve CD4+ T cells stimulated as indicated in the presence of different amounts of TGFβ for 5 days and then analyzed by FACS. D) qRT-PCR analysis of Blimp-1 mRNA expression in the same cells from experiment shown in (C). Data shown are from one experiment with pooled cells from three mice; similar results were obtained in 2 independent experiments.