. Author manuscript; available in PMC: 2013 Nov 25.

Published in final edited form as: Mol Cell Endocrinol. 2012 Aug 31;364(1-2):105–112. doi: 10.1016/j.mce.2012.08.015

Table 2.

Oligonucleotides used for site-directed mutagenesis. Mismatched nucleotides in each primer are underlined

Template Construct	Resulting construct	Forward primer, 5′ to 3′	Reverse primer, 5′ to 3′
pGL3c-141UTR	pGL3c-MutA1	GTAATAGTGAACATATGGAAAGTATTAGAAATAGGCCTTGTCTGTAAATACTGTAAATGCATTGGAATAA	TTATTCCAATGCATTTACAGTATTTACAGACAAGGCCTATTTCTAATACTTTCCATATGTTCAC TATTAC
pGL3c-MutA1	pGL3c-MutA12	TTTTTTTTGCCAAGGCTAATCCAATTATTATTATCATCGCGACCATAATTTATTTTGTCCATTGATGTATTTATTTTGTA	TACAAAATAAATACATCAATGGACAAAATAAATTATGGTCGCGATGATAATAATAATTGGATTAGCCTTGGCAAAAAAAA
pGL3c-MutA12	pGL3c-MutA123	CTAATCCAATTATTATTATCATCGCGACCATAACGCGTTTTGTCCATTGATGTATTTATTTTGTAAATGTAT	ATACATTTACAAAATAAATACATCAATGGACAAAACGCGTTATGGTCGCGATGATAATAATAATTGGATTAG
pGL3c-MutA123	pGL3c-MutA1234	CATAACGCGTTTTGTCCATTGATGTGCGCATTTTGTAAATGTATCTTGGTGCTG	CAGCACCAAGATACATTTACAAAATGCGCACATCAATGGACAAAACGCGTTATG
pGL3c-141UTR	pGL3c-MutA4	TTTACCATAATTTATTTTGTCCATTGATGTGCGCATTTTGTAAATGTATCTTGGTGCTGCTG	CAGCAGCACCAAGATACATTTACAAAATGCGCACATCAATGGACAAAATAAATTATGGTAAA
pGL3c-MutA4	pGL3c-MutA34	CTAATCCAATTATTATTATCACATTTACCATAACGCGTTTTGTCCATTGATGTGCGCATTTTGTAAAT	ATTTACAAAATGCGCACATCAATGGACAAAACGCGTTATGGTAAATGTGATAATAATAATTGG ATTAG
pGL3c-MutA34	pGL3c-MutA234	GCCAAGGCTAATCCAATTATTATTATCATCGCGACCATAACGCGTTTTGTCCATTGATGT	ACATCAATGGACAAAACGCGTTATGGTCGCGATGATAATAATAATTGGATTAGCCTTGGC