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. Author manuscript; available in PMC: 2012 Dec 23.
Published in final edited form as: J Cereb Blood Flow Metab. 2008 May 28;28(9):1597–1604. doi: 10.1038/jcbfm.2008.51

Figure 1.

Figure 1

Local measurements of oxygen partial pressure using a fine-tip oxygen microelectrode. (A) Tip of the microelectrode (2), mounted on a 3D-micromanipulator (3), is positioned in the center of frontal lens of the contact epiobjective (1) of the microscope. (B) Overview of the polarographic oxygen microelectrode used in the study. 2a—enlarged image of the copper wire fixed relative to a glass body using a small bit of quick-setting glue. 2b—photomicrograph of the microelectrode tip: the tip diameter including glass insulation, 4 μm; recess, 8 μm; scale, 3 μm. (C) Measurements of transmural PO2 gradient in dilated pial arteriole. Oxygen microelectrode (2) with a tip diameter of 4 μm and a recess of 10 μm and a glass micropipette (4) with a tip diameter of 27 μm is shown; scale, 50 μm. (D) The microelectrode tip is positioned on the outer arteriolar wall before applying the vasoactive agent. Arteriolar luminal diameter is 30 μm. (E) The microelectrode tip is positioned on the dilated arteriolar outer wall. Arteriolar luminal diameter is 46 μm; scale, 50 μm.