Skip to main content
. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Biomaterials. 2012 Nov 26;34(5):1471–1477. doi: 10.1016/j.biomaterials.2012.11.005

Fig. 6.

Fig. 6

(A) Sprouting was induced on one side of an ELL patterned in the center channel of a triple microchannel when 50ng/ml of VEGF was added to the right side channel to generate a VEGF gradient. VEGF gradient was started 24 hours after HUVEC seeding and maintained for 48 hours. Scale bar represents 50µm. (B) Fluorescent images of the wall of an ELL (top) and an ELL exposed to a VEGF gradient (bottom) stained for Jagged-1 (red) and nuclei (blue). Scale bar represents 50µm. (C) Quantitative analysis of endothelial sprout number, (D) sprout length, and (E) sprout area when ELLs were exposed to a variety of conditions. Error bars represent standard deviation.