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. Author manuscript; available in PMC: 2012 Dec 23.
Published in final edited form as: Mol Cell Neurosci. 2010 Jun 20;45(2):173–179. doi: 10.1016/j.mcn.2010.06.008

Fig. 1.

Fig. 1

Tyrosine phosphorylation of KCC2 occurs on two residues. A. HEK-293 cells were transfected with full-length KCC2 wild-type (WT), Y903F, Y1087F or Y903/1087F cDNA constructs. Cells were treated with sodium pervanadate (VO4, 100 µM, 30 min) followed by lysis and immunoprecipitation of KCC2. Immunoblotting with PY antibodies revealed the level of tyrosine phosphorylation of the different mutant constructs. Blots were also incubated with KCC2 antibodies to detect the total KCC2 level. In addition total extracts were immunoblotted with tubulin antibodies. Non-specific IgG was used as control for immunoprecipitation. B. The ratio of PY/KCC2 immunoreactivity was then determined and normalized to control. [*Significantly different from WT (p<0.01; value = mean ± SEM, Student's t-test, n=3)].