Skip to main content
. Author manuscript; available in PMC: 2012 Dec 23.
Published in final edited form as: Mol Cell Neurosci. 2010 Jun 20;45(2):173–179. doi: 10.1016/j.mcn.2010.06.008

Fig. 5.

Fig. 5

Muscarinic activation enhances tyrosine phosphorylation of KCC2 in cultured hippocampal neurons. A. Neurons were treated with carbachol (CCh, 100 µM) together with sodium pervanadate (VO4, 100 µM) for 30 min. Tyrosine phosphorylation of KCC2 was analyzed by immunoprecipitation of KCC2 followed by immunoblotting with PY antibody. Material was also immunoblotted with tubulin antibodies. The ratio of PY/KCC2 (B) and total KCC2 levels (C) were then measured and normalized to control. [*Significantly different from control condition (p<0.01; value = mean ± SEM, Student's t-test, n=3)].