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. Author manuscript; available in PMC: 2012 Dec 23.
Published in final edited form as: Pediatr Res. 2012 Sep 24;72(6):568–575. doi: 10.1038/pr.2012.124

Figure 2.

Figure 2

Overexpression of glutathione peroxidase 1 (GPx1) prevents hypoxic preconditioning (HPC)-mediated activation of cortical extracellular-regulated kinase (ERK). Phosphorylation of ERK was analyzed in cortical homogenates from wild-type (WT, white bars) and GPx1-overexpressing (black bars) animals exposed to preconditioning hypoxia at various time points after reoxygenation by western blotting. (a) Representative western blot of a complete time course of WT versus GPx1 transgenic (black) animals. Bar graph shows a summary of the results from densitometric analysis of bands from n ≥ 5 independent sets of animals. Phospho-ERK is expressed relative to ERK and to that of respective naive mice (=100%) ± SEM (*P < 0.05, one-way ANOVA). (b) Representative western blot of samples taken 0.5 h after reoxygenation. Bar graph represents the mean ± SEM of n = 10–11 animals (*P < 0.05, by one-way ANOVA) relative to respective naive controls. (c) Representative western blot of samples analyzed 24 h after HPC. Bar graph shows summary of densitometrically analyzed data from n = 5 animals ± SEM (*P < 0.05 by one-way ANOVA) relative to respective naive controls.