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. Author manuscript; available in PMC: 2012 Dec 23.
Published in final edited form as: Pediatr Res. 2012 Sep 24;72(6):568–575. doi: 10.1038/pr.2012.124

Figure 3.

Figure 3

Overexpression of glutathione peroxidase 1 (GPx1) prevents nuclear accumulation of activated (a) extracellular-regulated kinase (ERK) but not of (b) hypoxia-inducible factor (HIF)-1α. (a) Nuclear (black bars) and cytosolic (white bars) extracts prepared at 0.5 h after reoxygenation were analyzed for phospho-ERK (pERK) and total ERK by western blotting. HIF-1β was used as a nuclear marker protein. (Top) Representative western blot of one set of samples. (Bottom) Bar graph shows a summary of the results from densitometric analysis of bands from n = 5 independent sets of animals. Data are expressed relative to cytosolic level of pERK/ERK in respective naive controls (=100%) ± SEM. *P < 0.05, by one-way ANOVA. (b) Nuclear extracts prepared at 0 h after reoxygenation were analyzed for HIF-1α by western blotting. (Top) Representative western blot of HIF-1α analysis in nuclear extracts. (Bottom) Bar graph shows summary of results from densitometric analysis of HIF-1α band from n = 5 independent sets of animals. Data are expressed relative to wild-type (WT) hypoxic preconditioned (HPC) animals (=100%). No significant difference between WT HPC and GPx1 transgenic HPC by one-way ANOVA.